Publications

Method of the Year 2015

Nature Methods 13: 1 - 32 (2016)
Eisenstein M, Doerr A, Nogales E, Glaeser RM
Nature Methods has named single-particle cryo-electron microscppy the Method of the Year for 2015 based on “impressive leaps in achievable resolution” due to “recent technical advances, especially the development of direct-detection...

Structure and Membrane Remodeling Activity of ESCRT-III Helical Polymers

Science 350: 1548 - 1551 (2015)
McCullough J, Clippinger AK, Talledge N, Skowyra ML, Saunders MG, Naismith TV, Colf LA, Afonine P, Arthur C, Sundquist WI, Hanson PI, Frost A
A 4 Å cryo-EM reconstruction of a double-stranded helical copolymer of two different human ESCRT-III subunits. The cryo-EM data set was merged from multiple data sets acquired on several different microscopes, including a JEOL 3200FSC with a DE-12 camera.

Three-Dimensional Structural Characterization of HIV-1 Tethered to Human Cells

Journal of Virology 90: 1507 - 1521 (2015)
Strauss JD, Hammonds JE, Yi H, Ding L, Spearman P, Wright ER
Cryo-electron tomography (cryo-ET) of HIV-1 virus-like particles (VLPs) and virions tethered to human cells. Reconstructions showed the spatial arrangement and orientation of tetherin molecules at sites of HIV-1 restriction.

Automated batch fiducial-less tilt-series alignment in Appion using Protomo

Journal of Structural Biology In press: (2015)
Noble AJ, Stagg SM
A set of automated tilt-series alignment, contrast transfer function (CTF) correction, and reconstruction workflows for use in conjunction with the Appion/Leginon package, which are primarily targeted at automating structure determination with electron cryo-tomography.

The influence of frame alignment with dose compensation on the quality of single particle reconstructions

Journal of Structural Biology In press: (2015)
Spear JM, Noble AJ, Xie Q, Sousa DR, Chapman MS, Stagg SM
A 2.8 Å resolution cryo-EM structure of a recombinant gene therapy virus, AAV-DJ with Arixtra, from an FEI Titan Krios with a DE-20 Camera System. Several metrics are also presented for optimizing the compensation of beam damage and characterizing the factors that are limiting...

In Situ Study of Lithiation and Delithiation of MoS2 Nanosheets Using Electrochemical Liquid Cell Transmission Electron Microscopy

Topics: In situ TEM
Nano Letters 15: 5214 - 5220 (2015)
Zeng Z, Zhang X, Bustillo K, Niu K, Gammer C, Xu J, Zheng H
Observation of lithiation/delithiation of MoS2 nanosheets in a LiPF6/EC/DEC commercial electrolyte for the application of lithium-ion batteries using electrochemical liquid cell transmission electron microscopy (TEM) with a DE-12 Camera.

Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containment

AIMS Biophysics 2: 153-62 (2015)
Sherman M, Trujillo J, Bammes B, Jin L, Stumpf M, Weaver S
Tests of digital camera sensors (both CCD and CMOS direct detector) in a BSL-3 environment, and microscope performance after chlorine dioxide (ClO2) decontamination cycles.

A Newly Isolated Reovirus Has the Simplest Genomic and Structural Organization of Any Reovirus

Journal of Virology 89: 676 - 687 (2015)
Auguste AJ, Kaelber JT, Fokam EB, Guzman H, Carrington CV, Erasmus JH, Kamgang B, Popov VL, Jakana J, Liu X, Wood TG, Widen SG, Vasilakis N, Tesh RB, Chiu W, Weaver SC
The detection, genetic, phenotypic, and structural characteristics of a novel Dinovernavirus species isolated from mosquitoes collected in Cameroon, tentatively designated Fako virus (FAKV).

Catadioptric aberration correction in cathode lens microscopy

Topics: LEEM/PEEM
Ultramicroscopy 151: 191-8 (2015)
Tromp RM
A review of the use of electrostatic electron mirrors to correct the aberrations of the cathode lens objective lens in low energy electron microscope (LEEM) and photo electron emission microscope (PEEM) instruments.

Three-dimensional reconstructions of the bacteriophage CUS-3 virion reveal a conserved coat protein I-domain but a distinct tailspike receptor-binding domain

Virology 464-465: 55-66 (2014)
Parent KN, Tang J, Cardone G, Gilcrease EB, Janssen ME, Olson NH, Casjens SR, Baker TS
Icosahedrally averaged and asymmetric 3D cryo-EM reconstructions of the CUS-3 virion. The structure is compared to other P22-like phages.