An atomic model of brome mosaic virus using direct electron detection and real-space optimization
Nature Communications 5: 4808 (2014)
A rigorously-validated atomic model of brome mosaic virus (BMV) from single-particle cryo-EM using a DDD an damage compensation.
Crystal structure of a nematode-infecting virus
Proceedings of the National Academy of Sciences 111: 12781 - 12786 (2014)
The crystal structure and cryo-EM 3D reconstruction of an Orsay virus-like particle assembled from recombinant capsid protein (CP).
Improving signal to noise in labeled biological specimens using energy-filtered TEM of sections with a drift correction strategy and a direct detection device
Microscopy & Microanalysis 20: 706-714 (2013)
EFTEM imaging using a DDD with motion correction has a 3× improvement in signal compared to a similarly-formatted CCD.
Structure of the AcrAB-TolC multidrug efflux pump
Nature 509: 512-515 (2014)
The pseudo-atomic structure of a complete multidrug efflux pump in complex with a modulatory protein partner from E. coli.
Visualization of electrode-electrolyte interfaces in LiPF6/EC/DEC electrolyte for lithium ion batteries via in situ TEM
Nano Letters 14: 1745-1750 (2014)
The first published in situ TEM study with a DDD, showing direct visualization of electrochemical lithiation and delithiation of Au anodes.
Quantitative characterization of electron detectors for transmission electron microscopy
Journal of Structural Biology 184: 385-393 (2013)
A simple software tool for measuring MTF and DQE of electron detectors using a beam stop. DQE curves of various cameras are shown.
Allosteric regulation of DNA cleavage and sequence-specificity through run-on oligomerization
Structure 21: 1848-1858 (2013)
3D reconstructions of distinct populations of SgrAI oligomers, showing repeating helical structures in the presence of activating DNA.
Noise models and cryo-EM drift correction with a direct-electron camera
Ultramicroscopy 131: 61-69 (2013)
A filter that includes a model of noise from “hot” pixels is derived. This optimizes DDD motion correction by cross-correlation.
In silico, in vitro, and in vivo studies indicate the potential use of bolaamphiphiles for therapeutic siRNAs delivery
Molecular Therapy Nucleic Acids 2: e80 (2013)
Two different bolaamphiphiles are compared for their abilities to complex with siRNAs and deliver them into cells inducing gene silencing.
Movies of ice-embedded particles enhance resolution in electron cryo-microscopy
Structure 20: 1823-1828 (2012)
The first high-resolution cryo-EM reconstruction from a DDD, demonstrating the improvement in data quality from motion correction.