Since the introduction in 2008 of the DE-12 Camera as the first commercially-available direct detection TEM camera, the field of biological cryo-EM has seen an explosion of near-atomic resolution structures. Direct detection cameras have not only dramatically improved data quality, but they have also changed the entire paradigm of cryo-EM data collection.
Dramatically higher signal-to-noise ratio in each image means particles are easier to align and classify, and fewer particles are needed for reconstructions. With our cameras, you can signal all the way up to Nyquist frequency. This enables imaging at lower magnifications and increases the number of particles per image. Our cryo-EM cameras also feature our innovative movie-mode acquisition. The intrinsic dose fractionation enables motion correction of stage drift and beam-induced motion for a fluid and clear picture of particles and structures. Avoid long exposure times and reap the benefits of a gigantic field-of-view. Our DE-20 camera is nearly 40% larger than other direct detectors on the market, and our true 8k×8k DE-64 is nearly 5x larger. Our viewing fields also have better integrated signal across the entire frame in order to improve CTF correction and further boost the resolution of your 3D reconstructions.
Our unique combination of performance, throughput, and flexibility have enabled our customers to generate many single particle cryo-EM reconstructions at near-atomic resolution. But perhaps even more important than the absolute resolution (FSC value), reconstructions from DE cameras yield reliable, reproducible, de novo 3D reconstructions and atomic models. In fact, the atomic models from density maps generated from DE cameras have exceeded the quality of X-ray crystal structures of the same specimens (see Wang, et al., Nature Communications 5 (2014), 4808).
Our DE-Series Cameras enable the most efficient imaging conditions for any high-resolution cryo-EM. While some direct detectors require ultra-long exposure times to acquire decent images, our cameras deliver the best image in seconds. Not only does this speed up data collection, it also makes motion correction more effective. Users can fractionate their dose in much shorter time increments than other camera.
Since our cameras are designed to work optimally at nearly any beam brightness, you can collect images at low magnifications (for an even bigger field-of-view) without worrying about degrading image quality. This means highly-efficient imaging: lots of particles with little effort. In the highly competitive field of tomography, high throughput and productivity are absolutely crucial.
The high sensitivity and resolution of our DE-Series Cameras is also perfectly suited for tomography, where the total electron dose must be fractionated into a large number of very low-dose tilt-series images. Despite the low dose in each tilt image, our DE-Series Cameras allow users to visualize Thon rings to do CTF fitting in each tilt image. The exceptional contrast from our cameras produce stunning tomograms so you can clearly visualize the detail you need to see in your experiments.
Z-slices through a cryo-tomography reconstruction of HIV core pseudotypes with ASLV envelop Infected CV-1 cells. Data was collected on a DE-20 Camera System at 200 kV with motion correction for each tilt-series image. Courtesy of Greg Melikian, Mariana Marin, Cheri Hampton, and Elizabeth Wright at Emory University, Department of Pediatrics.