Low dose 3D-EM applications benefit from the high sensitivity and resolution of the DDD sensor
Integrated with SerialEM and Leginon for automated Cryo-EM or Cryo-Tomography data acquisition
Image of ice-embedded HK97 bacteriophage acquired at 300 keV on a JEOL 3200 microscope using SerialEM. Image magnification at 1.9 Angstrom/pixel. Total electron dose is 25 e-/A2. Image courtesy of NCMIR, UCSD
Epsilon 15 bacteriophage in ice. Image acquired on a 200kV JEOL 2010F microscope. Image magnification at 2.8 Angstrom/pixel. Total electron dose is 20 e-/A2. Image courtesy of NCMI, Baylor College of Medicine
Automated Multi-Scale Imaging of ice-embedded GroEL and TMV acquired at 120 keV FEI Tecnai microscope using Leginon. Image mag at 290X, 2,700X, and 21,000X. Dose in the final image is 11 e-/A2. Image courtesy of AMI, TSRI.
10 k x 7 k mosaic image from 9 individual images, 1 sec per image, mouse cerebellum, Courtesy Tom Deerinck, UCSD