DE-64 Camera System

DE-Series Cameras

Overview

Imagine the impact of having the performance of a direct detector and the power of “movie-mode” with the enormous field-of-view of photographic film…

Direct Electron is proud to announce our new DE-64 Camera System, the world’s first and only true 8k × 8k direct detector. The DE-64’s enormous 67.1 megapixel field-of-view is ideal for cryo-EM of very large viruses or cellular tomography, where a conventional camera may not capture a large enough area to be effective. Additionally, the ultra-large field-of-view dramatically decreases the number of images needed for each single particle experiment and improves 3D reconstructions by significantly improving CTF fitting.

The world’s first and only
true 8k × 8k direct detector.

The DE-64 features the 10th generation of our revolutionary Direct Detection Device (DDD®) sensor, delivering industry-leading resolution and flexible “movie-mode.” Although building an 8k direct detection sensor was challenging, we did not sacrifice performance by reducing the pixel size or features of the DE-64. The DE-64 has 6.5 μm pixels optimized for superior resolution (MTF) over a broad range of magnifications and exposure rates, it includes an integrated Faraday plate and 2k × 2k survey sensor, and it delivers user-adjustable full-frame streaming at up to 30 frames per second (fps).

CONTACT US now for more information or to add your facility to the list of the first DE-64 installations.

Direct Electron acknowledges the National Institutes of Health (USA) for supporting the development of the DE-64 under grant R44GM103417.

Highlights

DE-64 and DE-20 Cameras

All of our DE-series cameras deliver state-of-the-art performance and unique features that make them ideal for a wide range of TEM applications.

  • Direct detection of primary electrons—a revolutionary advancement in TEM data quality.
  • High signal-to-noise ratio (SNR) with consistent performance across all spatial frequencies and TEM magnifications.
  • Enormous 8k × 8k field-of-view. This direct detection camera is truly in a class by itself.
  • “Movie mode” provides high-speed acquisition of a continuous stream of frames.
  • Innovative movie-processing software further enhances data quality through drift correction, per-particle motion correction, radiation damage compensation, post-acquisition exposure setting, etc.
  • High performance for demanding applications, while maintaining ease-of-use and flexibility to maximize data collection efficiency.
  • Ultra high performance imaging in an elegantly designed, flexible, and easy-to-use camera system. Focus on generating results, not on the peculiarities of your camera.

Key Features

  • Enormous 8k × 8k field of view delivers 4x as much data with each exposure as a lesser 4k × 4k camera.
  • High-speed continuous streaming of raw frames, with flexible frame rate and exposure time.
  • Optional module to enable single electron counting.
  • Integrated near-axis 2k × 2k sur vey sensor.
  • Integrated Faraday plate for automated exposure measurement.
  • Integrated sensor protection cover.
  • Bottom mount or in JEOL film chamber.
  • Fully retractable with no sliding O-rings.
  • Open software architecture—fully extensible and easily integrated into custom workflows.
  • Complete access to raw data in industry-standard file formats.

Specifications

Detection electron energy rangeOptimized for 80 keV − 1.25 MeV
Pixel array specifications8192 × 8192 (67.1 MP) | 6.5 μm pixel pitch
Single-electron SNR~50:1 (300 kV)
Continuous frame-rate30 fps max, unbinned full-frame
Exposure rateConsistent performance at a broad range of exposure rates (e.g., 5 − 250 e-/pixel/s)
Mounting positionFully retractable | mounted on-axis TEM bottom port or in JEOL lm drawer
Survey sensorIntegrated near-axis 2048 × 2048 scintillator-coupled survey sensor
Sensor protectionIntegrated physical protection shutter | microscope blanking/shuttering | failsafe software
Computer systemCertified high-performance computer system with large, high-speed RAID array for data streaming
Acquisition & processing softwareConventional acquisition: DE-IM (full-featured, user-friendly) | μManager (free, open-source)

In situ movie acquisition: DE-StreamPix (continuous streaming)

Automated acquisition: Leginon | SerialEM | others using the DE SDK

“Movie” processing: DE image processing software (free, open-source, Python-based)

Note that the features and specifications shown above are typical, and are subject to change.

Ordering

CONTACT US now for more information or to add your facility to the list of the first DE-64 installations.